backgroundCorrect {limma}R Documentation

Correct Intensities for Background

Description

Background correct microarray expression intensities.

Usage

backgroundCorrect(RG, method="subtract", printer=RG$printer)

Arguments

RG an RGList object or a unclassed list containing the same components as an RGList
method character string specifying correction method. Possible values are "none", "subtract", "half", "minimum", "movingmin" or "edwards".
printer a list containing printer layout information, see PrintLayout-class

Details

If method="none" then the corrected intensities are equal to the foreground intensities, i.e., the background intensities are treated as zero. If method="subtract" then this function simply subtracts the background intensities from the foreground intensities which is the usual background correction method. If method="half" then any intensity which is less than 0.5 after background subtraction is reset to be equal to 0.5. If method="minimum" then any intensity which is zero or negative after background subtraction is set equal to half the minimum of the positive corrected intensities for that array. If method="movingmin" then the background estimates are replaced with the minimums of the backgrounds of the spot and its eight neighbors, i.e., the background is replaced by a moving minimum of 3x3 grids of spots. If method="edwards" a log-linear interpolation method is used to adjust lower intensities as in Edwards (2003).

Background correction (background subtraction) is also performed by the normalizeWithinArrays method for RGList objects, so it is not necessary to call backgroundCorrect directly unless one wants to use a method other than simple subtraction. Calling backgroundCorrect before normalizeWithinArrays will over-ride the default background correction.

Value

An RGList object in which components R and G are background corrected and components Rb and Gb are removed.

Author(s)

Gordon Smyth

References

Edwards, D. E. (2003). Non-linear normalization and background correction in one-channel cDNA microarray studies Bioinformatics 19, 825-833.

Yang, Y. H., Buckley, M. J., Dudoit, S., and Speed, T. P. (2002). Comparison of methods for image analysis on cDNA microarray data. Journal of Computational and Graphical Statistics 11, 108-136.

Yang, Y. H., Buckley, M. J., and Speed, T. P. (2001). Analysis of microarray images. Briefings in Bioinformatics 2, 341-349.

See Also

An overview of normalization and background correction functions is given in 4.Normalization.

Examples

RG <- new("RGList", list(R=c(1,2,3,4),G=c(1,2,3,4),Rb=c(2,2,2,2),Gb=c(2,2,2,2)))
backgroundCorrect(RG)
backgroundCorrect(RG, method="half")
backgroundCorrect(RG, method="minimum")

[Package limma version 1.6.7 Index]